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cyflow space device  (Partec)

 
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    Partec cyflow space device
    FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow <t>cytometry</t> (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.
    Cyflow Space Device, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyflow space device/product/Partec
    Average 90 stars, based on 1 article reviews
    cyflow space device - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Ultrahigh-Throughput Activity Engineering of Promiscuous Amidases through a Fluorescence-Activated Cell Sorting Assay"

    Article Title: Ultrahigh-Throughput Activity Engineering of Promiscuous Amidases through a Fluorescence-Activated Cell Sorting Assay

    Journal: ACS Catalysis

    doi: 10.1021/acscatal.5c01903

    FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow cytometry (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.
    Figure Legend Snippet: FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow cytometry (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.

    Techniques Used: Activity Assay, Fluorescence, Expressing, Negative Control, Flow Cytometry



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    Image Search Results


    FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow cytometry (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.

    Journal: ACS Catalysis

    Article Title: Ultrahigh-Throughput Activity Engineering of Promiscuous Amidases through a Fluorescence-Activated Cell Sorting Assay

    doi: 10.1021/acscatal.5c01903

    Figure Lengend Snippet: FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow cytometry (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.

    Article Snippet: Flow cytometry analysis was conducted with a Partec CyFlow space device (Sysmex Partec GmbH, Görlitz, Germany).

    Techniques: Activity Assay, Fluorescence, Expressing, Negative Control, Flow Cytometry

    Comparison of flow cytometry variables among groups

    Journal: Journal of Clinical Laboratory Analysis

    Article Title: Association of lymphocyte subsets with mortality in severe COVID‐19 pneumonia patients

    doi: 10.1002/jcla.24046

    Figure Lengend Snippet: Comparison of flow cytometry variables among groups

    Article Snippet: Multicolor flow cytometry device (PARTEK CYFLOW SPACE, Germany) and EXBIO antibody kits (Spain) were used for lymphocytes immunophenotyping.

    Techniques: Comparison, Flow Cytometry, Cell Counting

    Assessments of relationships between flow cytometry variables with ICU admission and mortality

    Journal: Journal of Clinical Laboratory Analysis

    Article Title: Association of lymphocyte subsets with mortality in severe COVID‐19 pneumonia patients

    doi: 10.1002/jcla.24046

    Figure Lengend Snippet: Assessments of relationships between flow cytometry variables with ICU admission and mortality

    Article Snippet: Multicolor flow cytometry device (PARTEK CYFLOW SPACE, Germany) and EXBIO antibody kits (Spain) were used for lymphocytes immunophenotyping.

    Techniques: Flow Cytometry